In the clip, which was released by CBS on Thursday afternoon, Oprah asks how she feels about the Palace hearing her “speak her truth”.
“I don’t know how they could expect that after all of this time we would still just be silent if there is an active role that the firm is playing in perpetuating falsehoods about us,” Meghan Markle says.
“And if that comes with risk of losing things, there is a lot that has been lost already.”
The tell-all interview, involving the former Meghan Markle and her husband, Prince Harry, comes amid bombshell allegations she bullied staff members during her time living at Kensington Palace.
The Times reported on Wednesday that Prince Harry and Meghan Markle’s communications assistant Jason Knauf had submitted a complaint against the new royal in 2018.
The complaint is said to have come after the Duchess allegedly “drove two personal assistants out of the royal household and undermined the confidence of a third staff member”.
A source quoted by The Sun described Kensington Palace as having a “tense atmosphere with staff occasionally reduced to tears”.
In a statement, a spokesperson for the Sussexes said Meghan denied all claims of bullying, but didn’t reject the assertion that a complaint had been made.
“The Duchess is saddened by this latest attack on her character, particularly as someone who has been the target of bullying herself and is deeply committed to supporting those who have experienced pain and trauma,” the statement said.
“She is determined to continue her work building compassion around the world and will keep striving to set an example for doing what is right and doing what is good.”
Harry and Meghan’s communications assistant Jason Knauf is reported to have emailed Prince William’s private secretary Simon Case after raising concerns that Meghan was bullying staff members with the Palace’s head of HR, Samantha Carruthers, according to The Sun.
In the October 2018 email, it is claimed Knauf said he believed the situation was “very serious” and Carruthers “agreed” with his concerns.
He wrote: “I am very concerned that the Duchess was able to bully two PAs out of the household in the past year.
“The treatment of X* was totally unacceptable.”
The shock bullying claims come just days before Prince Harry and Meghan’s highly-anticipated interview with Oprah Winfrey is set to air.
A teaser for the two-hour special shows Harry holding hands with wife Meghan at their Californian mansion while he speaks about his late mother, Princess Diana.
“For me, I’m just really relieved to be sitting here talking to you with my wife by my side, because I can’t begin to imagine what it must have been like for her going through this process by herself all those years ago,” Harry tells Winfrey as images of Diana flash across the screen.
“Because It’s been unbelievably tough for the two of us, but at least we have each other,” he continued.
Harry and Meghan’s sit-down with Oprah is set to air in the United States on Sunday March 7, which is midday Monday AEDT.
The two-hour special will see Winfrey question pregnant Meghan about everything “from stepping into life as a royal, marriage, motherhood, philanthropic work to how she is handling life under intense public pressure,” according to a statement.
Harry will then join the ladies, and the pair will tell Winfrey about “their move to the United States and their future hopes and dreams for their expanding family.”